Immunopotentiating nano-chitosan as potent vaccine carter for efficacious prophylaxis of filarial antigens.
Identifieur interne : 001C81 ( Main/Exploration ); précédent : 001C80; suivant : 001C82Immunopotentiating nano-chitosan as potent vaccine carter for efficacious prophylaxis of filarial antigens.
Auteurs : Balasubramaniyan Malathi [Inde] ; Santhanam Mona [Inde] ; Devasena Thiyagarajan [Inde] ; Perumal Kaliraj [Inde]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2015.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (immunologie), Agranulocytes (immunologie), Animaux, Antigènes d'helminthe (immunologie), Brugia malayi (immunologie), Chitosane (), Chitosane (immunologie), Filariose lymphatique (), Humains, Nanosphères (), Nanosphères (ultrastructure), Spectroscopie infrarouge à transformée de Fourier, Thiorédoxines (administration et posologie), Thiorédoxines (immunologie), Thiorédoxines (isolement et purification), Vaccins (), Vaccins (immunologie).
- MESH :
- administration et posologie : Thiorédoxines.
- immunologie : Activation des lymphocytes, Agranulocytes, Antigènes d'helminthe, Brugia malayi, Chitosane, Thiorédoxines, Vaccins.
- isolement et purification : Thiorédoxines.
- Animaux, Chitosane, Filariose lymphatique, Humains, Nanosphères, Spectroscopie infrarouge à transformée de Fourier, Vaccins.
English descriptors
- KwdEn :
- Animals, Antigens, Helminth (immunology), Brugia malayi (immunology), Chitosan (chemistry), Chitosan (immunology), Elephantiasis, Filarial (prevention & control), Humans, Leukocytes, Mononuclear (immunology), Lymphocyte Activation (immunology), Nanospheres (chemistry), Nanospheres (ultrastructure), Spectroscopy, Fourier Transform Infrared, Thioredoxins (administration & dosage), Thioredoxins (immunology), Thioredoxins (isolation & purification), Vaccines (chemistry), Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Thioredoxins.
- chemical , chemistry : Chitosan, Vaccines.
- chemical , immunology : Antigens, Helminth, Chitosan, Thioredoxins, Vaccines.
- chemistry : Nanospheres.
- immunology : Brugia malayi, Leukocytes, Mononuclear, Lymphocyte Activation.
- chemical , isolation & purification : Thioredoxins.
- prevention & control : Elephantiasis, Filarial.
- ultrastructure : Nanospheres.
- Animals, Humans, Spectroscopy, Fourier Transform Infrared.
Abstract
Lymphatic filariasis (LF), a morbid vector-borne parasitic infection affects millions in tropical areas. Complete eradication can only be achieved by the development of a potent vaccine. Among the various filarial antigens that have been characterized, antigens Brugia malayi thioredoxin (TRX) and abundant larval transcript (ALT) have produced recognizable level of protection in Jirds, thereby evidenced to be good vaccine candidates. In this study an attempt was made to enhance their immunoprophylactic activity by encapsulating them in natural polysaccharide chitosan forming nanospheres (CN). High encapsulation efficiency for TRX (93%) in CN (TCN) and ALT-2 (90%) in CN (ACN) was achieved. Morphological studies confirmed the spherical and uniform distribution of nanospheres to be 220 nm. The electrostatic interaction between chitosan and the antigens were confirmed using differential scanning calorimetry and FT-IR. The study revealed the immunostimulatory property of chitosan providing enhanced level of proliferation for encapsulated antigens in peripheral blood mononuclear cells from endemic normal personals, at low concentration (TCN mean stimulation index (SI)=4.23±0.15 and ACN (SI)=4.05±0.33) compared to stimulation obtained by antigens alone. Hence, our study demonstrated that natural macromolecule derived CN can be used as efficacious immunostimulatory vaccine carter for LF thereby diminishing pathological sequel.
DOI: 10.1016/j.ijbiomac.2014.11.014
PubMed: 25433130
Affiliations:
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Complete eradication can only be achieved by the development of a potent vaccine. Among the various filarial antigens that have been characterized, antigens Brugia malayi thioredoxin (TRX) and abundant larval transcript (ALT) have produced recognizable level of protection in Jirds, thereby evidenced to be good vaccine candidates. In this study an attempt was made to enhance their immunoprophylactic activity by encapsulating them in natural polysaccharide chitosan forming nanospheres (CN). High encapsulation efficiency for TRX (93%) in CN (TCN) and ALT-2 (90%) in CN (ACN) was achieved. Morphological studies confirmed the spherical and uniform distribution of nanospheres to be 220 nm. The electrostatic interaction between chitosan and the antigens were confirmed using differential scanning calorimetry and FT-IR. The study revealed the immunostimulatory property of chitosan providing enhanced level of proliferation for encapsulated antigens in peripheral blood mononuclear cells from endemic normal personals, at low concentration (TCN mean stimulation index (SI)=4.23±0.15 and ACN (SI)=4.05±0.33) compared to stimulation obtained by antigens alone. Hence, our study demonstrated that natural macromolecule derived CN can be used as efficacious immunostimulatory vaccine carter for LF thereby diminishing pathological sequel.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><country name="Inde"><noRegion><name sortKey="Malathi, Balasubramaniyan" sort="Malathi, Balasubramaniyan" uniqKey="Malathi B" first="Balasubramaniyan" last="Malathi">Balasubramaniyan Malathi</name></noRegion><name sortKey="Kaliraj, Perumal" sort="Kaliraj, Perumal" uniqKey="Kaliraj P" first="Perumal" last="Kaliraj">Perumal Kaliraj</name><name sortKey="Mona, Santhanam" sort="Mona, Santhanam" uniqKey="Mona S" first="Santhanam" last="Mona">Santhanam Mona</name><name sortKey="Thiyagarajan, Devasena" sort="Thiyagarajan, Devasena" uniqKey="Thiyagarajan D" first="Devasena" last="Thiyagarajan">Devasena Thiyagarajan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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